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pet28a expression vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pet28a expression vector
    Pet28a Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a+expression+vector/HindIII/10__1002_slash_cmtd__70098-243-14-32
    Average 99 stars, based on 10971 article reviews
    pet28a expression vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
    Article Snippet: .. The pool of DARPin variants from Round 4 was PCR amplified, cloned into the pET28a expression vector (using BamHI and HindIII restriction sites), and then transformed into chemically competent BL21(DE3) E. coli (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation.
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: The catalytic core of Leishmania donovani RECQ helicase unwinds a wide spectrum of DNA substrates and is stimulated by replication protein A.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.16153 This article is protected by copyright.. All rights reserved

    Amplification:

    Article Title: Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
    Article Snippet: .. The pool of DARPin variants from Round 4 was PCR amplified, cloned into the pET28a expression vector (using BamHI and HindIII restriction sites), and then transformed into chemically competent BL21(DE3) E. coli (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation.
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Clone Assay:

    Article Title: Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
    Article Snippet: .. The pool of DARPin variants from Round 4 was PCR amplified, cloned into the pET28a expression vector (using BamHI and HindIII restriction sites), and then transformed into chemically competent BL21(DE3) E. coli (NEB). ..

    Article Title: Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
    Article Snippet: .. The modified binB gene was then cloned into pET28a(+) expression vector using NdeI (NEB) and BamHI (NEB) restriction sites and T4 DNA ligase (NEB) and the construct was transformed into E. coli XL-10 gold competent cells under kanamycin (50 μg/ml) selection. .. Positively transformed colonies were selected through colony PCR using Taq DNA polymerase (Invitrogen).

    Article Title: Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin.
    Article Snippet: .. Therefore, we initially cloned mature MC1 (P23540; GI:133173) [10,11] into the pET28a(+) expression vector and introduced it into two E. coli SHuffle strains (NEB #3030 and #3026), which have been engineered to enhance oxidation and disulfide bond formation in cytoplasmically expressed proteins. ..

    Article Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli .
    Article Snippet: .. This was cloned into a pET28a expression vector using NcoI and NotI restriction enzymes (New England Biolabs), and the expression vector was transformed into E. coli BL21 (DE3) strain. ..

    Expressing:

    Article Title: Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
    Article Snippet: .. The pool of DARPin variants from Round 4 was PCR amplified, cloned into the pET28a expression vector (using BamHI and HindIII restriction sites), and then transformed into chemically competent BL21(DE3) E. coli (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: Binding Forces of Cellulose Binding Modules on Cellulosic Nanomaterials
    Article Snippet: All segments were obtained with PCR (using KAPA HiFi DNA Polymerase from KAPA Biosystems and primers from Eurofins Genomics), all flanked with BsaI restriction sites and appropriate 4bp overlaps. .. Using the Golden Gate cloning method2, the pieces were combined and ligated (with BsaI-HF and T4 DNA Ligase from NEB) into a pET28a (+) expression vector and transformed in chemically competent TOP10 E. coli cells. .. The resulting plasmid was obtained by miniprep (NucleoSpin Plasmid from Macherey-Nagel).

    Article Title: Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
    Article Snippet: .. The modified binB gene was then cloned into pET28a(+) expression vector using NdeI (NEB) and BamHI (NEB) restriction sites and T4 DNA ligase (NEB) and the construct was transformed into E. coli XL-10 gold competent cells under kanamycin (50 μg/ml) selection. .. Positively transformed colonies were selected through colony PCR using Taq DNA polymerase (Invitrogen).

    Article Title: Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin.
    Article Snippet: .. Therefore, we initially cloned mature MC1 (P23540; GI:133173) [10,11] into the pET28a(+) expression vector and introduced it into two E. coli SHuffle strains (NEB #3030 and #3026), which have been engineered to enhance oxidation and disulfide bond formation in cytoplasmically expressed proteins. ..

    Article Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli .
    Article Snippet: .. This was cloned into a pET28a expression vector using NcoI and NotI restriction enzymes (New England Biolabs), and the expression vector was transformed into E. coli BL21 (DE3) strain. ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation.
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: The catalytic core of Leishmania donovani RECQ helicase unwinds a wide spectrum of DNA substrates and is stimulated by replication protein A.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.16153 This article is protected by copyright.. All rights reserved

    Plasmid Preparation:

    Article Title: Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
    Article Snippet: .. The pool of DARPin variants from Round 4 was PCR amplified, cloned into the pET28a expression vector (using BamHI and HindIII restriction sites), and then transformed into chemically competent BL21(DE3) E. coli (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli .
    Article Snippet: .. This was cloned into a pET28a expression vector using NcoI and NotI restriction enzymes (New England Biolabs), and the expression vector was transformed into E. coli BL21 (DE3) strain. ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation.
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Transformation Assay:

    Article Title: Repurpose an FDA‐Approved Antibody Using DARPin‐Scaffolded Bridge Protein
    Article Snippet: .. The pool of DARPin variants from Round 4 was PCR amplified, cloned into the pET28a expression vector (using BamHI and HindIII restriction sites), and then transformed into chemically competent BL21(DE3) E. coli (NEB). ..

    Article Title: Binding Forces of Cellulose Binding Modules on Cellulosic Nanomaterials
    Article Snippet: All segments were obtained with PCR (using KAPA HiFi DNA Polymerase from KAPA Biosystems and primers from Eurofins Genomics), all flanked with BsaI restriction sites and appropriate 4bp overlaps. .. Using the Golden Gate cloning method2, the pieces were combined and ligated (with BsaI-HF and T4 DNA Ligase from NEB) into a pET28a (+) expression vector and transformed in chemically competent TOP10 E. coli cells. .. The resulting plasmid was obtained by miniprep (NucleoSpin Plasmid from Macherey-Nagel).

    Article Title: Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
    Article Snippet: .. The modified binB gene was then cloned into pET28a(+) expression vector using NdeI (NEB) and BamHI (NEB) restriction sites and T4 DNA ligase (NEB) and the construct was transformed into E. coli XL-10 gold competent cells under kanamycin (50 μg/ml) selection. .. Positively transformed colonies were selected through colony PCR using Taq DNA polymerase (Invitrogen).

    Article Title: Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli .
    Article Snippet: .. This was cloned into a pET28a expression vector using NcoI and NotI restriction enzymes (New England Biolabs), and the expression vector was transformed into E. coli BL21 (DE3) strain. ..

    Ligation:

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Article Title: Functional Characterization of MIP_07528 of Mycobacterium indicus pranii for Tyrosine Phosphatase Activity Displays Sensitivity to Oxidative Inactivation and Plays a Role in Immunomodulation.
    Article Snippet: .. The amplified PCR products and the pET28a expression vector were digested with BamHI and HindIII restriction enzymes (NEB), followed by ligation using T4 DNA ligase (NEB). ..

    Cloning:

    Article Title: Binding Forces of Cellulose Binding Modules on Cellulosic Nanomaterials
    Article Snippet: All segments were obtained with PCR (using KAPA HiFi DNA Polymerase from KAPA Biosystems and primers from Eurofins Genomics), all flanked with BsaI restriction sites and appropriate 4bp overlaps. .. Using the Golden Gate cloning method2, the pieces were combined and ligated (with BsaI-HF and T4 DNA Ligase from NEB) into a pET28a (+) expression vector and transformed in chemically competent TOP10 E. coli cells. .. The resulting plasmid was obtained by miniprep (NucleoSpin Plasmid from Macherey-Nagel).

    Modification:

    Article Title: Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
    Article Snippet: .. The modified binB gene was then cloned into pET28a(+) expression vector using NdeI (NEB) and BamHI (NEB) restriction sites and T4 DNA ligase (NEB) and the construct was transformed into E. coli XL-10 gold competent cells under kanamycin (50 μg/ml) selection. .. Positively transformed colonies were selected through colony PCR using Taq DNA polymerase (Invitrogen).

    Construct:

    Article Title: Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
    Article Snippet: .. The modified binB gene was then cloned into pET28a(+) expression vector using NdeI (NEB) and BamHI (NEB) restriction sites and T4 DNA ligase (NEB) and the construct was transformed into E. coli XL-10 gold competent cells under kanamycin (50 μg/ml) selection. .. Positively transformed colonies were selected through colony PCR using Taq DNA polymerase (Invitrogen).

    Article Title: The catalytic core of Leishmania donovani RECQ helicase unwinds a wide spectrum of DNA substrates and is stimulated by replication protein A.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.16153 This article is protected by copyright.. All rights reserved

    Selection:

    Article Title: Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
    Article Snippet: .. The modified binB gene was then cloned into pET28a(+) expression vector using NdeI (NEB) and BamHI (NEB) restriction sites and T4 DNA ligase (NEB) and the construct was transformed into E. coli XL-10 gold competent cells under kanamycin (50 μg/ml) selection. .. Positively transformed colonies were selected through colony PCR using Taq DNA polymerase (Invitrogen).

    Recombinant:

    Article Title: The catalytic core of Leishmania donovani RECQ helicase unwinds a wide spectrum of DNA substrates and is stimulated by replication protein A.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/FEBS.16153 This article is protected by copyright.. All rights reserved



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